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Retroviral-mediated gene transfer into hepatocytes in vivo.

Ferry, N; Duplessis, O; Houssin, D; Danos, O; Heard, JM; (1991) Retroviral-mediated gene transfer into hepatocytes in vivo. Proc Natl Acad Sci U S A , 88 (19) pp. 8377-8381. 10.1073/pnas.88.19.8377.

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Abstract

Stable gene transfer into hepatocytes might be used to compensate for a genetic deficiency affecting liver function or to deliver diffusible factors into the blood stream. In rats, we have combined retroviral-mediated gene transfer with a surgical procedure in which the liver is temporarily excluded from the circulation and infected in vivo. Partial hepatectomy was performed 24-48 hr before perfusion with virus to induce hepatocyte division and facilitate viral integration. A helper-free recombinant retrovirus coding for beta-galactosidase with nuclear localization was used to score cells that expressed the transgene. For at least 3 months after gene transfer, up to 5% of hepatocytes expressed nuclear beta-galactosidase. Whereas in vitro reimplantation of genetically modified hepatocytes has proved to be inefficient in stably transferring genes into the liver, our approach provides a feasible alternative.

Type: Article
Title: Retroviral-mediated gene transfer into hepatocytes in vivo.
Location: United States
DOI: 10.1073/pnas.88.19.8377
Keywords: Animals, Base Sequence, Cell Compartmentation, Gene Expression, Genetic Vectors, Leukemia Virus, Murine, Liver, Liver Regeneration, Molecular Sequence Data, Oligonucleotides, Perfusion, Polymerase Chain Reaction, Rats, Rats, Inbred Lew, Recombinant Fusion Proteins, Regulatory Sequences, Nucleic Acid, Transfection, beta-Galactosidase
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/90968
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