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The structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose

Thompson, D; Pepys, MB; Tickle, I; Wood, S; (2002) The structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose. J MOL BIOL , 320 (5) 1081 - 1086. 10.1016/S0022-2836(02)00514-4.

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Abstract

Two monoclinic (P2(1)) crystal forms of human serum amyloid P component (SAP) in complex with the 4,6-pyruvate acetal of beta-D-galactose (MObetaDG) were prepared. Structure analysis by molecular replacement and refinement at 2.2 Angstrom resolution revealed that crystal form 1 (a = 95.76 Angstrom, b = 70.53 Angstrom, c = 103.41 Angstrom, beta = 96.80degrees) contained a pentamer in the asymmetric unit with a structure very similar to that of the published search model. The mode of ligand co-ordination was also similar except that four of the five subunits showed bound ligand with an additional H-bond between O1 of the galactose and the side-chain of Lys79. One sub-unit showed no bound ligand and a vacant calcium site close to a crystal contact. The 2.6 Angstrom resolution structure of crystal form 2 (a = 118.60 Angstrom, b = 109.10 Angstrom, c = 120.80 Angstrom and beta = 95.16degrees) showed ten sub-units in the asymmetric unit, all with two bound calcium ions and ligand. The most extensive protein-protein interactions between pentamers describe an AB face-to-face interaction involving 15 ion pairs that sandwiches five molecules of bound MObetaDG at the interface. (C) 2002 Elsevier Science Ltd. All rights reserved.

Type: Article
Title: The structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose
DOI: 10.1016/S0022-2836(02)00514-4
Keywords: serum amyloid P component, methyl 4,6-O-(L-carboxyethylidene)-beta-D-galactopyranoside, amyloid, decamer, crystal structure, C-REACTIVE PROTEIN, SYSTEMIC AMYLOIDOSIS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/90714
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