Al-Mufti, RAM and Pedley, RB and Marshall, D and Begent, RHJ and Hilson, A and Winslet, MC and Hobbs, KEF (1999) In vitro assessment of Lipiodol-targeted radiotherapy for liver and colorectal cancer cell lines. BRIT J CANCER , 79 (11-12) 1665 - 1671.
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Intra-arterial Lipiodol has been used to deliver targeted therapies to primary, and some metastatic, liver cancers. Targeted radiotherapy has been used by substituting the iodine in Lipiodol with (131)Iodine (I-131). Early clinical results are encouraging, but the variable response may partly depend on local pharmacokinetics. This study evaluated the in vitro cytotoxic effects of I-131-Lipiodol on human hepatocellular carcinoma (Hep-G2), human colorectal metastatic cancer (SW620), human colorectal hepatic cancer (LoVo) and human umbilical vein endothelia[ cells (HUVEC) cell lines. The cell cultures were exposed to I-131-Lipiodol for 48 h, following which cell counts and viability were assessed by haemocytometer, S-Rhodamine uptake and radioactivity assay. The effect of exposure to control Lipiodol, I-131-Lipiodol and I-131 alone was evaluated. I-131-Lipiodol was cytotoxic against all the cancer cell lines but not against the non-malignant (HUVEC) cell line. The cytotoxicity effects were Very similar in all the cancer cell lines. There were no cytotoxic effects following exposure to plain I-131 in any of the cell lines (malignant and non-malignant). A similar trend was seen with radioactivity counts using a gamma counter. The cytotoxic effect of I-131-lipiodol had a graded effect with an increase in cytotoxicity following the increase in the radioactive dose. This study showed that there was a marked cytotoxic effect by I-131-Lipiodol on all the cancer cell lines. There was no difference between the controls and the (131)Iodine. This suggests that effective I-131-Lipiodol targeted therapy is dependent on the uptake and retention of Lipiodol by malignant cells.
|Title:||In vitro assessment of Lipiodol-targeted radiotherapy for liver and colorectal cancer cell lines|
|Keywords:||lipiodol therapy, targeted radiotherapy, primary liver cancer, colorectal liver metastases, in-vitro cytotoxicity, HEPATIC-ARTERY INJECTION, HEPATOCELLULAR-CARCINOMA, ENDOTHELIAL-CELLS, I-131-LABELED LIPIODOL, DRUG, METASTASES, ANTIGEN|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)|
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Oncology
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