UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Axoplasmic importins enable retrograde injury signaling in lesioned nerve

Hanz, S; Perlson, E; Willis, D; Zheng, JQ; Massarwa, R; Huerta, JJ; Koltzenburg, M; ... Fainzilber, M; + view all (2003) Axoplasmic importins enable retrograde injury signaling in lesioned nerve. NEURON , 40 (6) 1095 - 1104.

Warning

A more recent version of this eprint is available. Click here to view it.

Full text not available from this repository.

Abstract

Axoplasmic proteins containing nuclear localization signals (NLS) signal retrogradely by an unknown mechanism in injured nerve. Here we demonstrate that the importin/karyopherin alpha and beta families underlie this process. We show that importins are found in axons at significant distances from the cell body and that importin beta protein is increased after nerve lesion by local translation of axonal mRNA. This leads to formation of a high-affinity NLS binding complex that traffics retrogradely with the motor protein dynein. Trituration of synthetic NLS peptide at the injury site of axotomized dorsal root ganglion (DRG) neurons delays their regenerative outgrowth, and NLS introduction to sciatic nerve concomitantly with a crush injury suppresses the conditioning lesion induced transition from arborizing to elongating growth in L4/L5 DRG neurons. These data suggest a model whereby lesion-induced upregulation of axonal importin beta may enable retrograde transport of signals that modulate the regeneration of injured neurons.

Type: Article
Title: Axoplasmic importins enable retrograde injury signaling in lesioned nerve
Keywords: P75 NEUROTROPHIN RECEPTOR, NUCLEAR TRANSPORT FACTOR, PROTEIN-SYNTHESIS, APLYSIA NEURONS, GROWTH CONES, IN-VIVO, AXON, LOCALIZATION, REGENERATION, ALPHA
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: http://discovery.ucl.ac.uk/id/eprint/8529

Available Versions of this Item

Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item