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Affinity purified human antiphospholipid antibodies bind normal term placenta

Donohoe, S; Kingdom, JCP; Mackie, IJ; (1999) Affinity purified human antiphospholipid antibodies bind normal term placenta. LUPUS , 8 (7) 525 - 531.

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Abstract

Antiphospholipid antibodies (aPL) are associated with an increased incidence of fetal loss, but the pathophysiology remains unclear. One mechanism may involve the binding of aPL directly to the placenta where they may initiate placental thrombosis and infarction. We have developed an immunofluorescent technique to detect human aPL binding to human placenta. Endogenous immunoglobulins were eluted by extensive washing and residual staining was prevented by incorporating multiple blocking steps. APL were affinity purified on both cardiolipin and phosphatidylserine liposomes from the sera of six patients with aPL (five antiphospholipid syndrome (,APS) patients and one post bone marrow transplant patient). Heterogeneous binding to normal term placenta, involving either the trophoblast microvillous surface, stromal and perivascular regions was demonstrated by affinity purified aPL from five of six patients. Preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting studies have demonstrated that aPL bind a number of placental proteins. beta(2)GPI was not the predominant protein bound by aPL using this technique. This study provides further evidence for the involvement of aPL in mediating placental damage.

Type: Article
Title: Affinity purified human antiphospholipid antibodies bind normal term placenta
Keywords: antiphospholipid antibodies, placental proteins, immunofluorescence, beta(2)-glycoprotein-I, SYSTEMIC LUPUS-ERYTHEMATOSUS, ANTICARDIOLIPIN ANTIBODIES, DECIDUAL VASCULOPATHY, FETAL LOSS, PREGNANCY, CELLS, WOMEN, ANTICOAGULANT, THROMBOSIS, EXPRESSION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/85200
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