UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Development of the mouse retinal vasculature: angiogenesis versus vasculogenesis

Fruttiger, M.; (2002) Development of the mouse retinal vasculature: angiogenesis versus vasculogenesis. Investigative Ophthalmology and Visual Science , 43 (2) pp. 522-527.

Full text not available from this repository.

Abstract

Purpose: The inner vasculature of the retina develops as a spreading network, which is preceded by spindle-shaped cells. These cells are alleged to be vascular precursor cells (angioblasts). This study was designed to test whether such angioblasts exist in neonatal mouse retina. Methods: In situ hybridization and immunohistochemistry on mouse retinal wholemount preparations were used to visualize specific vascular cell types. Results: In situ hybridization with an RNA probe against vascular endothelial growth factor receptor (VEGFR)-2 (a marker for endothelial cells and angioblasts) labeled the vascular network but failed to label the spindle-shaped cells in front of it. A probe against VEGFR1, a marker for endothelial cells only, revealed the same staining pattern. Pericytes, visualized with a probe against platelet-derived growth receptor (PDGFR)-ß, were spread over the entire vessel network, but not beyond it. However, in situ hybridization with a probe against PDGFR{alpha} (a marker for retinal astrocytes) labeled spindle-shaped cells preceding the vessel network. Conclusions: These observations imply that in the mouse retina the spindle-shaped cells preceding the forming vasculature are immature retinal astrocytes and not vascular precursor cells and that the primary vascular network in the retina develops by angiogenesis (budding from existing vessels) and not vasculogenesis (assembly of dispersed angioblasts).

Type:Article
Title:Development of the mouse retinal vasculature: angiogenesis versus vasculogenesis
Publisher version:http://www.iovs.org/cgi/content/abstract/43/2/522
Language:English
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology

Archive Staff Only: edit this record