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Reduced cholesteryl ester transfer in plasma of patients with lipoprotein lipase deficiency

Bagdade, JD; Ritter, MC; Lithell, H; Bassett, D; Mailly, F; Talmud, P; (1996) Reduced cholesteryl ester transfer in plasma of patients with lipoprotein lipase deficiency. J LIPID RES , 37 (8) 1696 - 1703. Gold open access

Abstract

The net mass transfer of cholesteryl ester (CE) from high density lipoprotein (HDL) to the apolipoprotein (apo) B-containing lipoproteins, very low density lipoprotein (VLDL) and low density lipoprotein (LDL) in plasma (cholesteryl ester transfer (CET)) from three patients lacking lipoprotein lipase (LpL) activity was significantly lower (P < 0.001) than in plasma from fasting control subjects with comparable triglyceride levels. Chylomicrons isolated from LpL-deficient fasting plasma showed the same low level of CET activity as observed in the intact plasma when combined with HDL and cholesteryl ester transfer protein (CETP)-containing d 1.063 g/ml bottom fractions from control subjects. Preincubation of chylomicrons and large triglyceride-rich lipoproteins (S-f > 400) from LpL-deficient plasma with milk LpL, however, stimulated the capacity to engage in CET 4- to 5-fold to the same level as chylomicrons and VLDL from control subjects after a fat load. Consistent with these measurements of CET activity in plasma, chylomicrons obtained from the LpL-deficient subjects after a 14-h fast had higher TG/CE ratios than chylomicrons from controls 3 h after ingesting a fat load (LpL-deficient 26.3 +/- 9.0 vs. controls 6.9 +/- 2.1; mean +/- SD). The mass of CETP did not differ in LpL-deficient and control subjects (LpL-deficient 1.03 +/- 0.22 mu g/ml vs. controls 1.58 +/- 0.58 mu g/ml). These studies are consistent with earlier in vitro studies showing that the actions of lipoprotein lipase and its lipolytic products are essential for maximal cholesteryl ester transfer protein activity.

Type:Article
Title:Reduced cholesteryl ester transfer in plasma of patients with lipoprotein lipase deficiency
Open access status:An open access publication
Publisher version:http://www.jlr.org/papbyrecent.shtml
Keywords:lipoprotein lipase, chylomicrons, VLDL, cholesteryl ester transfer protein, DEPENDENT DIABETES-MELLITUS, HIGH-DENSITY-LIPOPROTEINS, TRANSFER PROTEIN, ABNORMALITIES, MECHANISMS, LIPEMIA
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science

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