JOSE, PJ and COLLINS, PD and PERKINS, JA and BEAUBIEN, BC and TOTTY, NF and WATERFIELD, MD and HSUAN, J and WILLIAMS, TJ (1991) IDENTIFICATION OF A 2ND NEUTROPHIL-CHEMOATTRACTANT CYTOKINE GENERATED DURING AN INFLAMMATORY REACTION IN THE RABBIT PERITONEAL-CAVITY INVIVO - PURIFICATION, PARTIAL AMINO-ACID-SEQUENCE AND STRUCTURAL RELATIONSHIP TO MELANOMA-GROWTH-STIMULATORY ACTIVITY. BIOCHEM J , 278 493 - 497.
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The intraperitoneal injection of zymosan in the rabbit results in the generation of an inflammatory exudate containing oedema-forming and chemoattractant activities. Previous studies demonstrated the early appearance of the complement fragment C5a, followed by the generation of two mediators related to the cytokine interleukin-8 that were separable by cation-exchange h.p.l.c. N-Terminal amino acid sequencing identified one of these mediators as rabbit interleukin-8. This paper describes the purification of the second cytokine by cation-exchange, gel-filtration and reversed-phase h.p.l.c. The purified material had both oedema-forming and chemoattractant activity when assayed in rabbit skin in vivo. On SDS/PAGE a single 6-8 kDa band was observed and N-terminal amino acid sequencing of the reduced and alkylated protein positively identified 36 amino acids. This sequence revealed the rabbit homologue of melanoma-growth-stimulatory activity. The identification of these two cytokines in vitro will provide an opportunity to investigate the importance of their co-release in the inflammatory process.
|Title:||IDENTIFICATION OF A 2ND NEUTROPHIL-CHEMOATTRACTANT CYTOKINE GENERATED DURING AN INFLAMMATORY REACTION IN THE RABBIT PERITONEAL-CAVITY INVIVO - PURIFICATION, PARTIAL AMINO-ACID-SEQUENCE AND STRUCTURAL RELATIONSHIP TO MELANOMA-GROWTH-STIMULATORY ACTIVITY|
|Keywords:||VASCULAR-PERMEABILITY, IN-111 NEUTROPHILS, INTERLEUKIN-8, ACCUMULATION, PRETREATMENT, MONOCYTES, INVITRO, CELLS, SKIN|
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