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Cord blood progenitor cells have greater transendothelial migratory activity and increased responses to SDF-1 and MIP-3 beta compared with mobilized adult progenitor cells

Yong, K; Fahey, A; Reeve, L; Nicholls, C; Thomas, NSB; Pizzey, A; Ings, SJ; ... Linch, DC; + view all (1999) Cord blood progenitor cells have greater transendothelial migratory activity and increased responses to SDF-1 and MIP-3 beta compared with mobilized adult progenitor cells. BRIT J HAEMATOL , 107 (2) 441 - 449.

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Abstract

When cord blood is used as a source of haemopoietic stem cells for transplantation, fewer cells are required per kg of recipient. This greater engraftment efficiency of cord brood cells may relate to an increased ability to traverse sinusoidal endothelium, a crucial step in the homing of stem cells. We report that freshly isolated cord blood progenitors migrated more efficiently than mobilized adult cells. Cord blood progenitors responded rapidly to growth factor stimulation with an increase in migratory ability within 24 h whereas mobilized adult cells responded only after 72 h (P < 0.01). Cord blood cells also exited G(0)/G(1) rapidly; after 24 h of growth factor exposure, 20.2 +/- 1.2% of cord blood CD34(+) cells were in S + G(2)/M compared to 6.9 +/- 1.2% of adult CD34(+) cells (P < 0.01). Proliferating CFC migrated more efficiently (13.3 +/- 3.4% for GM-CFC) than non-proliferating CFC (1.4 +/- 0.5%, P < 0.01) as determined using a H-3-thymidine suicide assay. Cord blood progenitor cells also demonstrated a greater transmigratory response to chemokine stimulation compared with adult cells; this was manifested as a differential response of freshly isolated cells to SDP-1, and of growth factor activated cells to MIP-3 beta. Finally, cord blood CD34(+) cells express higher levels of the chemokine receptor for SDF-1, CXCR4, when compared with mobilized adult CD34(+) cells (P < 0.05).

Type: Article
Title: Cord blood progenitor cells have greater transendothelial migratory activity and increased responses to SDF-1 and MIP-3 beta compared with mobilized adult progenitor cells
Keywords: cord blood, migration, chemokine, endothelium, INTEGRIN-MEDIATED ADHESION, PERIPHERAL-BLOOD, HEMATOPOIETIC PROGENITORS, GROWTH-FACTORS, BONE-MARROW, TRANSPLANTATION, MECHANISMS, ACTIVATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/84099
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