UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

T-CELLS FROM NORMAL AND MYASTHENIC INDIVIDUALS RECOGNIZE THE HUMAN ACETYLCHOLINE-RECEPTOR - HETEROGENEITY OF ANTIGENIC SITES ON THE ALPHA-SUBUNIT

MELMS, A; MALCHEREK, G; GERN, U; WIETHOLTER, H; MULLER, CA; SCHOEPFER, R; LINDSTROM, J; (1992) T-CELLS FROM NORMAL AND MYASTHENIC INDIVIDUALS RECOGNIZE THE HUMAN ACETYLCHOLINE-RECEPTOR - HETEROGENEITY OF ANTIGENIC SITES ON THE ALPHA-SUBUNIT. ANN NEUROL , 31 (3) 311 - 318.

Full text not available from this repository.

Abstract

The alpha-subunit of the nicotinic acetylcholine receptor is the major target of the autoimmune response in myasthenia gravis. We investigated the proliferative response of T cells from patients with myasthenia gravis and healthy volunteers to recombinant polypeptides of the human acetylcholine receptor including the full-length alpha-subunit (alpha-1-437). T cells from 20 (7 1%) of 28 patients and 7 (37%) of 19 healthy volunteers responded in primary cultures. Subsequently, specific T-cell lines were established: CD4+, CD8-, UCHL1+, and major histocompatibility complex (MHC) class II-restricted. Using a set of fragments of the alpha-subunit, major antigenic sites could be localized on the extracellular, N-terminal part of the molecule as well as close to the C-terminus. The T-cell response was heterogeneous, both among different individuals and among T-cell lines from a single donor. These T cells did not cross-react with Torpedo acetylcholine receptor, which was previously used as a substitute for human muscle acetylcholine receptor, suggesting that the T cells had a bias for unique human sequences. A single antigenic fragment could be presented in the context of different MHC class II molecules, and different fragments could be presented in the context of the same MHC molecule. This supports earlier observations of considerable heterogeneity in dealing with acetylcholine receptor as an autoantigen on the level of both T cells and antigen-presenting cells. The data also demonstrate that acetylcholine receptor-specific T cells are present in the normal immune repertoire, and emphasize the role of immune regulation for maintaining a state of tolerance.

Type: Article
Title: T-CELLS FROM NORMAL AND MYASTHENIC INDIVIDUALS RECOGNIZE THE HUMAN ACETYLCHOLINE-RECEPTOR - HETEROGENEITY OF ANTIGENIC SITES ON THE ALPHA-SUBUNIT
Keywords: CELLULAR IMMUNE-RESPONSE, MAIN IMMUNOGENIC REGION, SYNTHETIC PEPTIDES, LYMPHOCYTES-T, PROLIFERATIVE RESPONSES, HEALTHY-INDIVIDUALS, LINE TE671, CLASS-II, GRAVIS, SPECIFICITY
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/83649
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item