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Biosynthesis and functions of eicosanoids generated by the coelomocytes of the starfish, Asterias rubens

Pope, EC; Taylor, GW; Rowley, AF; (2007) Biosynthesis and functions of eicosanoids generated by the coelomocytes of the starfish, Asterias rubens. COMP BIOCHEM PHYS B , 147 (4) 657 - 666. 10.1016/j.cbpb.2007.04.006.

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Abstract

Eicosanoids are a group of oxygenated fatty acid derivatives formed from C20 polyunsaturated fatty acids, including arachidonic and eicosapentaenoic acids. The potential of the coelomocytes of the starfish, Asterias rubens, to generate eicosanoids through the cyclooxygenase (COX) and lipoxygenase (LOX) pathways was investigated using reverse-phase high performance liquid chromatography, enzyme immunoassay and gas chromatography-mass spectrometry. The principal LOX product was identified as 8-hydroxyeicosatetracnoic acid (8-HETE) with 8-hydroxyeicosapentaenoic acid (8-REPE) synthesised at significantly lower levels. No classical prostaglandins (PG), such as PGE2 or PGD2, were found to be generated by ionophore-challenged coelomocytes. Incubation of coelomocytes with lipopolysaccharides from either Escherichia coli or Salmonella abortus failed to induce an increase in generation of LOX products and the presence of 8-HETE (0-25 mu M) had no significant effect on the in vitro phagocytic activity of Asterias coelomocytes. Neither indomethacin (a COX inhibitor) or esculetin (a LOX inhibitor) had any effect on the clearance of the bacterium, Vibrio splendidus, from the coelontic cavity of starfish suggesting that products of these enzymes are not involved in such coelomocyte responses to foreign particles. (c) 2007 Elsevier Inc. All rights reserved.

Type: Article
Title: Biosynthesis and functions of eicosanoids generated by the coelomocytes of the starfish, Asterias rubens
DOI: 10.1016/j.cbpb.2007.04.006
Keywords: starfish, eicosanoids, prostaglandins, phagocytosis, mass spectrometry, cellular defence reactions, STRONGYLOCENTROTUS-PURPURATUS, MARINE EICOSANOIDS, MOLECULAR-CLONING, SEA-URCHIN, CYCLOOXYGENASE, IDENTIFICATION, ACIDS, INVERTEBRATES, PHAGOCYTOSIS, ACTIVATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/82191
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