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DISSOCIATION OF RECA FILAMENTS FROM DUPLEX DNA BY THE RUVA AND RUVB DNA-REPAIR PROTEINS

ADAMS, DE; TSANEVA, IR; WEST, SC; (1994) DISSOCIATION OF RECA FILAMENTS FROM DUPLEX DNA BY THE RUVA AND RUVB DNA-REPAIR PROTEINS. P NATL ACAD SCI USA , 91 (21) 9901 - 9905.

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Abstract

The RuvA and RuvB proteins of Escherichia coli act late in recombination and DNA repair to catalyze the branch migration of Holliday junctions made by RecA. In this paper, we show that addition of RuvAB to supercoiled DNA that is bound by RecA leads to the rapid dissociation of the RecA nucleoprotein filament, as determined by a topological assay that measures DNA underwinding and a restriction endonuclease protection assay. Disruption of the RecA filament requires RuvA, RuvB, and hydrolysis of ATP. These findings suggest several important roles for the RuvAB helicase during genetic recombination and DNA repair: (i) displacement of RecA filaments from double-stranded DNA, (ii) interruption of RecA-mediated strand exchange, (iii) RuvAB-catalyzed branch migration, and (iv) recycling of RecA protein.

Type:Article
Title:DISSOCIATION OF RECA FILAMENTS FROM DUPLEX DNA BY THE RUVA AND RUVB DNA-REPAIR PROTEINS
Keywords:RECOMBINATION, BRANCH MIGRATION, HELICASE, TOPOISOMERASE, DNA SUPERCOILING, ESCHERICHIA-COLI RUVA, SYNTHETIC HOLLIDAY JUNCTIONS, STRAND EXCHANGE, BRANCH MIGRATION, RECOMBINATION, PURIFICATION, HELICASE, INVITRO, BINDING
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences

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