Huggett, JF;
Novak, T;
Garson, JA;
Green, C;
Morris-Jones, SD;
Miller, RF;
Zumla, A;
(2008)
Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon.
BMC Research Notes
, 1
, Article 70. 10.1186/1756-0500-1-70.
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Abstract
PCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e. that the reaction of interest and the control reaction are equally susceptible to inhibition. To test this assumption we performed inhibition assessment on DNA extracts from human urine samples, fresh urine and EDTA using different PCR reactions.
| Type: | Article |
|---|---|
| Title: | Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon |
| Location: | England |
| Identifier: | PMCID: PMC2564953 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/1756-0500-1-70 |
| Publisher version: | http://dx.doi.org/10.1186/1756-0500-1-70 |
| Language: | English |
| Additional information: | © 2008 Huggett et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health > Infection and Population Health |
| URI: | https://discovery.ucl.ac.uk/id/eprint/80337 |
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