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The deoxyribonucleic acid modification enzyme of bacteriophage P1.

Brockes, JP; Brown, PR; Murray, K; (1972) The deoxyribonucleic acid modification enzyme of bacteriophage P1. Biochem J , 127 (1) pp. 1-10. 10.1042/bj1270001.

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Abstract

The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20-24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0-6.25: the apparent K(m) for S-adenosyl-l-methionine is 5x10(-6)m.

Type: Article
Title: The deoxyribonucleic acid modification enzyme of bacteriophage P1.
Location: England
DOI: 10.1042/bj1270001
Keywords: Ammonium Sulfate, Catalase, Centrifugation, Zonal, Chromatography, DEAE-Cellulose, Coliphages, DNA, Viral, Dialysis, Hydrogen-Ion Concentration, Hydrolysis, Methylation, Methyltransferases, Morpholines, Purines, S-Adenosylmethionine, Sulfonic Acids, Tritium
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/77967
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