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Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release

Ciccolini, LAS; Shamlou, PA; Titchener-Hooker, NJ; Ward, JM; Dunnill, P; (1998) Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release. BIOTECHNOL BIOENG , 60 (6) 768 - 770.

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Abstract

SDS-alkaline lysis of recombinant Escherichia coil cell suspensions was carried out in a coaxial cylinder rheometer, and the data were used to establish the time course of lysis reaction. The results of the experiments showed that cell lysis reaction time depended on cell strain but was unaffected by plasmid size and plasmid copy number. The high molecular weight globular proteins and chromosomal DNA were denatured, and the resulting changes in rheometric measurements characterised the denaturation time. (C) 1998 John Wiley & Sons, Inc.

Type: Article
Title: Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release
Keywords: SDS-alkaline lysis process, lysis reaction time, chromosomal DNA denaturation, shear damage of DNA
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Engineering Science Faculty Office
URI: http://discovery.ucl.ac.uk/id/eprint/77831
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