Boren, J and Lookene, A and Makoveichuk, E and Xiang, SQ and Gustafsson, M and Liu, HQ and Talmud, P and Olivecrona, G (2001) Binding of low density lipoproteins to lipoprotein lipase is dependent on lipids but not on apolipoprotein B. J BIOL CHEM , 276 (29) 26916 - 26922.
Lipoprotein lipase (LPL) efficiently mediates the binding of lipoprotein particles to lipoprotein receptors and to proteoglycans at cell surfaces and in the extracellular matrix. It has been proposed that LPL increases the retention of atherogenic Lipoproteins in the vessel wall and mediates the uptake of lipoproteins in cells, thereby promoting lipid accumulation and plaque formation. We investigated the interaction between LPL and low density lipoproteins (LDLs) with special reference to the protein-protein interaction between LPL and apolipoprotein B (apoB). Chemical modification of lysines and arginines in apoB or mutation of its main proteoglycan binding site did not abolish the interaction of LDL with LPL as shown by surface plasmon resonance (SPR) and by experiments with THP-I macrophages. Recombinant LDL with either apoB100 or apoB48 bound with similar affinity. In contrast, partial delipidation of LDL markedly decreased binding to LPL. In cell culture experiments, phosphatidylcholine-containing liposomes competed efficiently with LDL for binding to LPL. Each LDL particle bound several (up to 15) LPL dimers as determined by SPR and by experiments with THP-I macrophages. A recombinant NH2-terminal fragment of apoB (apoB17) bound with low affinity to LPL as shown by SPR, but this interaction was completely abolished by partial delipidation of apoB17. me conclude that the LPL-apoB interaction is not significant in brid,bing LDL to cell surfaces and matrix components; the main interaction is between LPL and the LDL lipids.
|Title:||Binding of low density lipoproteins to lipoprotein lipase is dependent on lipids but not on apolipoprotein B|
|Open access status:||An open access publication|
|Keywords:||TO-RETENTION HYPOTHESIS, N-TERMINAL 17-PERCENT, SMOOTH-MUSCLE-CELLS, HEPARAN-SULFATE, RECEPTOR-BINDING, IN-VIVO, ATHEROSCLEROSIS, EXPRESSION, SURFACE, PROTEOGLYCANS|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science|
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