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Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart

Fauchon, MAC; Pell, TJ; Baxter, GF; Yellon, DM; Latchman, DS; Hubank, MF; Mayne, LV; (2005) Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart. EXP MOL MED , 37 (4) 311 - 322. Gold open access

Abstract

Preconditioning of the myocardium rapidly induces a number of transcription factors, which are likely to be responsible for a cascade of transcriptional changes underlying the development of delayed adaptation. Identifying. these changes provides insight into the molecular pathways elicited by sub-lethal ischaemia and the mechanism leading to delayed adaptation. Genes up-regulated in rabbit myocardium in vivo by ischaemic preconditioning following reperfusion for 2 h, 4 h and 6 h post treatment were identified by representational difference analysis of cDNA (cDNA. RDA). The area of the left ventricle rendered ischaemic by preconditioning or the equivalent area of sham-treated animals was isolated and cDNA.RDA performed. Three novel genes and six genes with known function where identified, including the TGF beta receptor interacting protein 1, the a isoform of the A subunit of PP2 and the cap binding protein NCBP1. To determine whether expression of these genes correlated with preconditioning per se, expression was measured in myocardium after both ischaemic as well as heat shock induced preconditioning following 2 h, 4 h, and 6 h reperfusion. These genes were induced in rabbit myocardium in vivo by both ischaemia and heat shock, consistent with a fundamental role in the development of delayed adaptation. The well described role of PP2 in modulating the mitogen-activated protein kinase pathway and promoting cell survival is consistent with our previous work, which identified the reperfusion injury salvage kinase pathway in mediating the protective effects of ischaemic preconditioning. Expression of Trip1 and Ncbp1 also implicates TGF beta signalling pathways and RNA processing and transport in delayed adaptation to stress in the myocardium.

Type: Article
Title: Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart
Open access status: An open access publication
Publisher version: http://www.e-emm.org/current.htm
Keywords: gene expression profiling, heat-shock response, ischemic preconditioning, myocardial, myocardial ischemia, myocardium, reperfusion, PROTEIN PHOSPHATASE 2A, GROWTH-FACTOR-BETA, SERINE/THREONINE PHOSPHATASES, MYOCARDIAL-INFARCTION, MAP KINASE, RAT HEARTS, ISCHEMIA, REPERFUSION, ACTIVATION, APOPTOSIS
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute of Cardiovascular Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH - Directors Office
URI: http://discovery.ucl.ac.uk/id/eprint/75017
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