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Gene Delivery into Neuronal Cells by Calcium Phosphate-Mediated Transfection

Watson, A; Latchman, D; (1996) Gene Delivery into Neuronal Cells by Calcium Phosphate-Mediated Transfection. Methods , 10 (3) pp. 289-291.

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Abstract

This paper describes a method for introducing DNA constructs into primary adult dorsal root ganglion (DRG) neuron cultures. The method uses a modified calcium phosphate technique and enables relatively small numbers of cells to be used. We have used this method to study the promoter sequences responsible for mediating gene activity and nerve growth factor responsiveness in DRG neurons. It can also be used, however, for other purposes such as testing the effect on neuronal function of overexpressing a specific gene product.

Type: Article
Title: Gene Delivery into Neuronal Cells by Calcium Phosphate-Mediated Transfection
Location: United States
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH - Directors Office
URI: http://discovery.ucl.ac.uk/id/eprint/74645
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