THEORY OF DNA ELECTROPHORESIS IN PHYSICAL GELS AND ENTANGLED POLYMER-SOLUTIONS.
PHYS REV E
2408 - 2416.
A scaling theory is presented for the electrophoretic mobility of DNA in sieving media that form dynamically evolving meshworks, such as physical gels and solutions of entangled polymers. In such media, the topological constraints on the DNA's motion are perpetually changing as cross links break and rejoin or as the polymers diffuse. It is shown that if the rate of constraint release falls within a certain range (which depends on the field strength), fractionation can be extended to higher molecular weights than would be feasible using a permanent gel of equivalent pore size. This improvement is a consequence of the disruptive effect that constraint release has on the mechanism of molecular orientation. Numerical simulations support the predictions of the theory. The possibility of realizing such a system in practice, with the aim of improving on current electrophoresis methods, is commented upon. It is suggested that semidilute polymer solutions may be a versatile medium for the rapid separation of long single-stranded DNA molecules, and the particular quality of solution required is identified.
|Title:||THEORY OF DNA ELECTROPHORESIS IN PHYSICAL GELS AND ENTANGLED POLYMER-SOLUTIONS|
|Keywords:||CAPILLARY ELECTROPHORESIS, POLYACRYLAMIDE, MOBILITY|
|UCL classification:||UCL > School of BEAMS
UCL > School of BEAMS > Faculty of Maths and Physical Sciences
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