MacDonald, AJ; Libri, NA; Lustigman, S; Barker, SJ; Whelan, MA; Semper, AE; Rosenberg, WM; (2008) A novel, helminth-derived immunostimulant enhances human recall responses to hepatitis C virus and tetanus toxoid and is dependent on CD56(+) cells for its action. CLIN EXP IMMUNOL , 152 (2) 265 - 273. 10.1111/j.1365-2249.2008.03623.x.
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We have described previously an immunostimulant derived from Onchocerca volvulus, the helminth parasite that causes onchocerciasis. Recombinant O. volvulus activation-associated secreted protein-1 (rOv-ASP-1) was a potent adjuvant for antibody and cellular responses to protein, polypeptide and small peptide antigens. Our aims were to determine whether rOv-ASP-1 is immunostimulatory for human peripheral blood mononuclear cells (PBMC) and, if so, whether it could augment cellular responses against human pathogen antigens in vitro. Cytokines from rOv-ASP-1-stimulated human PBMC were measured by a fluorescence activated cell sorter-based multiplex assay. Recall responses of normal healthy donor (NHD) and chronic hepatitis C virus (c-HCV)-infected patient PBMC to tetanus toxoid (TT) or HCV core (HCVco) antigen, respectively, were measured by interferon-gamma enzyme-linked immunospot assays. Interferon-gamma was the predominant cytokine induced by rOv-ASP-1. 77.3% of NHD anti-TT and 88.9% of c-HCV anti-HCVco responses were enhanced by rOv-ASP-1. The immunostimulant effect was dependent upon contact between CD56(+) and CD56(-) fractions of PBMC. We have described a helminth-derived protein that can act as an immunostimulant for human recall responses in vitro to TT and, perhaps more importantly, HCV antigens in patients with chronic HCV infection. Our longer-term goal would be to boost anti-viral responses in chronic infections such as HCV.
|Title:||A novel, helminth-derived immunostimulant enhances human recall responses to hepatitis C virus and tetanus toxoid and is dependent on CD56(+) cells for its action|
|Keywords:||adjuvant, helminth, hepatitis C, NK cell, Onchocerca volvulus, SECRETED PROTEIN, ONCHOCERCA-VOLVULUS, NECATOR-AMERICANUS, T-CELLS, NK CELLS, VACCINE, ACTIVATION, INFECTION, PARASITE, ANTIGEN|
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