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Expression of a novel cytokine, IL-4deltaf in HIV and HIV-tuberculosis co-infection

Dheda, K; Chang, JS; Breen, RAM; Haddock, JA; Lipman, MC; Kim, LU; Huggett, JF; ... Zumla, A; + view all (2005) Expression of a novel cytokine, IL-4deltaf in HIV and HIV-tuberculosis co-infection. AIDS , 19 (15) 1601 - 1606.

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Abstract

Background: Correcting the Th2 shift in HIV/AIDS represents a potential intervention strategy. However data on interleukin (IL)-4 expression in HIV or AIDS are uninterpretable because of failure to distinguish between IL-4 and its splice variant and natural antagonist, IL-4 delta 2.Objective: To determine Th1 [interferon (IFN)-gamma], IL-4 delta 2 and Th2 (IL-4) expression in whole blood and lung lavage from healthy volunteers and in HIV or HIV-tuberculosis (TB) co-infection.Design: Cross-sectional with prospective cohort.Methods: Expression of IL-4 delta 2, IL-4 and IFN-gamma were determined by quantitative real-time PCR, using unstimulated cells from whole blood and lung lavage, in 20 HIV-TB (pulmonary) co-infected patients, 20 matched HIV-positive controls and 20 HIV-negative healthy volunteers. Results were correlated with plasma viral load, CD4 cell counts, radiological scores and response to anti-TB treatment.Results: Compared to HIV negative donors, stable HIV-positive donors did not have increased levels of mRNA encoding IL-4, IL-4 delta 2 or IFN-gamma in blood or lavage. By contrast, the HIV-TB co-infected donors had increased IL-4 and IFN-gamma in both compartments. However the antagonist, IL-4 delta 2 was increased only in lavage. Consequently the dominant form was IL-4 delta 2 in lavage, but IL-4 itself in blood. The lung IL-4/IFN-gamma ratio correlated with radiological disease extent. With anti-TB treatment, IL-4 levels did not change whilst IL-4 delta 2 levels increased significantly.Conclusions: IL-4 and its natural antagonist, IL-4 delta 2 and are not upregulated in the absence of opportunistic infection. However in HIV-TB co-infection both cytokines increase in lung, but only IL-4 in the periphery. Further studies are required to determine if IL-4 facilitates systemic HIV progression. (c) 2005 Lippincott Williams & Wilkins

Type: Article
Title: Expression of a novel cytokine, IL-4deltaf in HIV and HIV-tuberculosis co-infection
Keywords: Th1/Th2 cells, tuberculosis, HIV, IL-4, IL-4 delta 2, IMMUNODEFICIENCY-VIRUS-INFECTION, ALTERNATIVE SPLICE VARIANT, CD4(+) T-CELLS, MYCOBACTERIUM-TUBERCULOSIS, MESSENGER-RNA, HUMAN IL-4, IN-VITRO, INTERLEUKIN-4, IL-4-DELTA-2, INDIVIDUALS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: http://discovery.ucl.ac.uk/id/eprint/7181
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