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Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice

Kahn, E; Lizard, G; Dumas, D; Frouin, F; Menetrier, F; Stoltz, JF; Todd-Pokropek, A; (2004) Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice. ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY , 26 (4) 233 - 238.

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Abstract

OBJECTIVE: To characterize the behavior of magneto-fluorescent products injected in mice intravenously.STUDY DESIGN: The magnetic resonance imaging (MRI). products were labelled with fluorescent molecules to examine the biodistribution process in vivo and observe them at the cellular level by means of confocal microscopy. Three-dimensional (3D) sequences of images were obtained by spectral analysis of sample preparations in a multiphoton confocal microscope and analyzed by the factor analysis of medical image sequence algorithm, which provides factor curves. Factor images are the result of image-processing methods that utilize information from emission spectra. Preparations are also screened in the counting mode to provide fluorescent lifetime imaging microscopy (FLIM) characterizations.RESULTS: Factor images and FLIM images can help to analyze MRI targeting inside the liver and thoracic aorta of mice. They show positive detection of Fe-Texas red and BOPTA-Eu in the liver and positive detection Of Fe-Texas red and negative detection of BOPTA-Eu inside the thoracic aorta.CONCLUSION: This investigation established the utility of fluorescent MRI contrast agents as in vivo staining tools for cellular sites.

Type:Article
Title:Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice
Location:Montpellier, FRANCE
Keywords:magnetic resonance imaging, contrast media, fluorescent dyes, mouse, laboratory, liver, aorta, thoracic, HUMAN-PAPILLOMAVIRUS DNA, CYTOGENETIC PREPARATIONS, CONFOCAL ANALYSIS, SEQUENCES, MICROSCOPY, EUROPIUM, NUCLEI, CELLS, HELA
UCL classification:UCL > School of BEAMS > Faculty of Engineering Science > Medical Physics and Bioengineering

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