Funes, JM and Quintero, M and Henderson, S and Martinez, D and Qureshi, U and Westwood, C and Clements, MO and Bourboulia, D and Pedley, RB and Moncada, S and Boshoff, C (2007) Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production. P NATL ACAD SCI USA , 104 (15) 6223 - 6228. 10.1073/pnas.0700690104.
An increased dependency on glycolysis for ATP production is considered to be a hallmark of tumor cells. Whether this increase in glycolytic activity is due mainly to inherent metabolic alterations or to the hypoxic microenvironment remains controversial. Here we have transformed human adult mesenchymal stem cells (MSC) using genetic alterations as described for differentiated cells. Our data suggest that MSC require disruption of the same pathways as have been shown for differentiated cells to confer a fully transformed phenotype. Furthermore, we found that MSC are more glycolytic than primary human fibroblasts and, in contrast to differentiated cells, do not depend on increased aerobic glycolysis for ATP production during transformation. These data indicate that aerobic glycolysis (the Warburg effect) is not an intrinsic component of the transformation of adult stem cells, and that oncogenic adaptation to bioenergetic requirements, in some circumstances, may also rely on increases in oxidative phosphorylation. We did find, however, a reversible increase in the transcription of glycolytic enzymes in tumors generated by transformed MSC, indicating this is a secondary phenomenon resulting from adaptation of the tumor to its microenvironment.
|Title:||Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production|
|Open access status:||An open access publication|
|Publisher version:||http://www.ncbi.nlm.nih.gov/pmc/ articles/PMC1851087/?tool=pubmed|
|Keywords:||adult stem cells, glycolysis, Warburg effect, HUMAN TUMOR-CELLS, CANCER-CELLS, AEROBIC GLYCOLYSIS, NUDE-MICE, METABOLISM, GROWTH, CARCINOGENESIS, MECHANISMS, APOPTOSIS, ELEMENTS|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Cancer Biology|
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Oncology
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