Pichaud, F and Roux, S and Frendo, JL and Delage-Mourroux, R and Maclouf, J and de Vernejoul, MC and Moukhtar, MS and Jullienne, A (1997) 1,25-dihydroxyvitamin D3 induces NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase in human neonatal monocytes. Blood , 89 (6) 2105 - 2112.
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1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] induces the differentiation of monocytes into macrophage-like cells in vitro. To identify the genes expressed during this process, we performed differential display polymerase chain reaction on RNA extracted from cord blood monocytes (CBMs) treated with 1,25-(OH)2D3. Treated CBMs expressed type-I 15-hydroxyprostaglandin dehydrogenase (type-I 15-PGDH), the key enzyme of prostaglandin E2 (PGE2) catabolism and a 15-PGDH-related mRNA (15-PGDHr). This newly described 15-PGDH-related mRNA was constitutively expressed in adult monocytes. 15-PGDH gene(s) transcription was accompanied by the appearance of the 15-PGDH activity in treated CBMs. In addition, the cyclooxygenase 2 mRNA level was decreased and PGE2 levels in the culture mediums were lowered (50%). Our results stress that 1,25-(OH)2D3, at least in neonatal monocytes, can exert, directly or indirectly, a dual control on key enzymes of PGE2 metabolism. In conclusion, we suggest that modifications in prostaglandin metabolism, induced by the expression of type-I 15-PGDH and the downregulation of cyclooxygenase 2, could be involved in monocytic differentiation.
|Title:||1,25-dihydroxyvitamin D3 induces NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase in human neonatal monocytes.|
|Keywords:||Adult, Calcitriol, Culture Media, Cyclooxygenase 2, Dinoprostone, Enzyme Activation, Enzyme Induction, Fetal Blood, Humans, Hydroxyprostaglandin Dehydrogenases, Isoenzymes, Membrane Proteins, Monocytes, NAD, Polymerase Chain Reaction, Prostaglandin-Endoperoxide Synthases, RNA, Messenger|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Laboratory for Molecular Cell Biology|
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