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QUANTITATION OF PLASMA MEVALONIC ACID USING GAS-CHROMATOGRAPHY ELECTRON-CAPTURE MASS-SPECTROMETRY

SCOPPOLA, A; MAHER, VMG; THOMPSON, GR; RENDELL, NB; TAYLOR, GW; (1991) QUANTITATION OF PLASMA MEVALONIC ACID USING GAS-CHROMATOGRAPHY ELECTRON-CAPTURE MASS-SPECTROMETRY. J LIPID RES , 32 (6) 1057 - 1060. Gold open access

Abstract

Circulating concentrations of mevalonic acid (MVA) change in parallel with, and may be used as a marker of, cholesterol biosynthesis. Plasma MVA levels have been quantified using a sensitive and specific capillary gas chromatography-electron capture mass spectrometric assay. The detection limit for MVA in plasma is 100 pg/ml; in intra-assay variation is 5.11%; the inter-assay variation is 7.7%. Using this assay, the mean plasma MVA in 15 normolipidemic subjects was 2.37 +/- 1.2 ng/ml (range 0.41-5.31 ng/ml). Administration of 40 mg of simvastatin (an HMG-CoA reductase inhibitor) significantly accentuated the diurnal decrease in plasma MVA levels.This assay may be useful in investigating cholesterol synthesis rates in different dyslipidemias and individual responses to HMG-CoA reductase-inhibiting drugs.

Type: Article
Title: QUANTITATION OF PLASMA MEVALONIC ACID USING GAS-CHROMATOGRAPHY ELECTRON-CAPTURE MASS-SPECTROMETRY
Open access status: An open access publication
Publisher version: http://www.jlr.org/papbyrecent.shtml
Keywords: CHOLESTEROL BIOSYNTHESIS, DIURNAL RHYTHM, HMG-COA REDUCTASE, ELECTRON CAPTURE IONIZATION, HETEROZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA, CHOLESTEROL-SYNTHESIS, URINE, RAT
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/69216
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