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Efficient delivery of human single fiber-derived muscle precursor cells via biocompatible scaffold

Boldrin, L; Malerba, A; Vitiello, L; Cimetta, E; Piccoli, M; Messina, C; Gamba, PG; ... De Coppi, P; + view all (2008) Efficient delivery of human single fiber-derived muscle precursor cells via biocompatible scaffold. CELL TRANSPLANT , 17 (5) 577 - 584.

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Abstract

The success of cell therapy for skeletal muscle disorders depends upon two main factors: the cell source and the method of delivery. In this work we have explored the therapeutic potential of human muscle precursor cells (hMPCs), obtained from single human muscle fibers, implanted in vivo via micropatterned scaffolds. hMPCs were initially expanded and characterized in vitro by immunostaining and flow cytometric analysis. For in vivo studies, hMPCs were seeded onto micropatterned poly-lactic-glycolic acid 3D-scaffolds fabricated using soft-lithography and thermal membrane lamination. Seeded scaffolds were then implanted in predamaged tibialis anterior muscles of CDI nude mice; hMPCs were also directly injected in contralateral limbs as controls. Similarly to what we previously described with mouse precursors cells, we found that hMPCs were able to participate in muscle regeneration and scaffol d-i mpl anted muscles contained a greater number of human nuclei, as revealed by immunostaining and Western blot analyses. These results indicate that hMPCs derived from single fibers could be a good and reliable cell source for the design of therapeutic protocols and that implantation of cellularized scaffolds is superior to direct injection for the delivery of myogenic cells into regenerating skeletal muscle.

Type: Article
Title: Efficient delivery of human single fiber-derived muscle precursor cells via biocompatible scaffold
Keywords: human single fibers, muscle precursor cells, biocompatible scaffold, muscle regeneration, SATELLITE CELLS, SKELETAL-MUSCLE, STEM-CELLS, SOFT LITHOGRAPHY, TRANSPLANTATION, MATRIX, REGENERATION, EXPRESSION, DYSTROPHIN, MYOBLASTS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Development Bio and Cancer Prog
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Developmental Neurosciences Prog
URI: http://discovery.ucl.ac.uk/id/eprint/65899
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