UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Expression of gonadotropin-releasing hormone type-I (GnRH-I) and type-II (GnRH-II) in human peripheral blood mononuclear cells (PMBCs) and regulation of B-lymphoblastoid cell proliferation by GnRH-I and GnRH-II

Tanriverdi, F; Gonzalez-Martinez, D; Silveira, LFG; Hu, Y; Maccoll, GS; Travers, P; Bouloux, PMG; (2004) Expression of gonadotropin-releasing hormone type-I (GnRH-I) and type-II (GnRH-II) in human peripheral blood mononuclear cells (PMBCs) and regulation of B-lymphoblastoid cell proliferation by GnRH-I and GnRH-II. EXP CLIN ENDOCR DIAB , 112 (10) 587 - 594. 10.1055/s-2004-830404.

Full text not available from this repository.

Abstract

GnRH-I and its receptor (GnRHR-I) have previously been demonstrated and shown to be biologically active in the immune system, notably within T cells. Recently however a second form of GnRH (GnRH-II) has been described in the human. The function of both these neuropeptides in B lymphocytes has not previously been explored. The present study investigates GnRH-l and GnRH-II expression in human peripheral mononuclear blood cells (PMBCs) and B lymphoblastoid cells (B-LCLs), as well as their action in regulating B-LCL proliferation in the presence and absence of interleukin-2 (IL-2), both in GnRHR-I mutated lymphocytes and in a normal control. RT-PCR and immunocytochemistry identified locally produced GnRH-l and GnRH-II in all cell groups. Treatment of normal B-LCLs with GnRH-l (10(-9) M and 10(-5) M) or with interleukin-2 (IL-2) (50 IU/ml) resulted in a significant increase in cell proliferation compared with the untreated control. IL-2 and GnRH-I (10(-1) M, 10(-6) M, 10(-5) M) induced greater proliferation in normal B-LCLs than IL-2 treatment alone. No significant proliferation occurred in GnRHR-I defective B-LCLs, in response to either GnRH-l (10(-9) and 10-5 M) or IL-2 treatment, nor to IL-2 and GnRH-l (10(-10) to 10(-5) M) co-treatment when compared to controls. Co-incubation of IL-2 and IL-2 + GnRH 10(-5) M with a GnRH antagonist (Cetrorelix; 10(-6) M) significantly attenuated the proliferation in normal B-LCLs. GnRH-II did not affect proliferation of normal B-LCLs alone, and did not alter the proliferative response to IL-2. Further investigation is required to clarify the physiological relevance of local GnRH-I/GnRH-II in immune system responsiveness.

Type: Article
Title: Expression of gonadotropin-releasing hormone type-I (GnRH-I) and type-II (GnRH-II) in human peripheral blood mononuclear cells (PMBCs) and regulation of B-lymphoblastoid cell proliferation by GnRH-I and GnRH-II
DOI: 10.1055/s-2004-830404
Keywords: GnRH-I, GnRH-II, B lymphocytes, proliferation, interleukin-2, MESSENGER-RIBONUCLEIC-ACID, GENE-EXPRESSION, BINDING-SITES, RECEPTOR, ANTAGONIST, LYMPHOCYTES, ANALOGS, THYMUS, INTERLEUKIN-2, HYPOTHALAMUS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Metabolism and Experi Therapeutics
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health
URI: http://discovery.ucl.ac.uk/id/eprint/65822
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item