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Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription

Alic, N; Ayoub, N; Landrieux, E; Favry, E; Baudouin-Cornu, P; Riva, M; Carles, C; (2007) Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription. P NATL ACAD SCI USA , 104 (25) 10400 - 10405. 10.1073/pnas.0704116104.

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Abstract

We examine here the mechanisms ensuring the fidelity of RNA synthesis by RNA polymerase III (Pol III). Misincorporation could only be observed by using variants of Pol III deficient in the intrinsic RNA cleavage activity. Determination of relative rates of the reactions producing correct and erroneous transcripts at a specific position on a tRNA gene, combined with computational methods, demonstrated that Pol III has a highly efficient proofreading activity increasing its transcriptional fidelity by a factor of 10(3) over the error rate determined solely by selectivity (1.8 x 10(-4)). We show that Pot III slows down synthesis past a misincorporation to achieve efficient proofreading. We discuss our findings in the context of transcriptional fidelity studies performed on RNA Pols, proposing that the fidelity of transcription is more crucial for Pol III than Pol II.

Type: Article
Title: Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription
DOI: 10.1073/pnas.0704116104
Keywords: nucleotide selectivity, transcriptional fidelity, ELONGATION-FACTOR TFIIS, SACCHAROMYCES-CEREVISIAE, IN-VIVO, SUBSTRATE-SPECIFICITY, HYDROLYTIC CLEAVAGE, ESCHERICHIA-COLI, CHAIN ELONGATION, DNA-REPLICATION, MECHANISM, TERMINATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: http://discovery.ucl.ac.uk/id/eprint/65481
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