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A NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT (ALPHA-7) IS DEVELOPMENTALLY REGULATED AND FORMS A HOMOOLIGOMERIC CHANNEL BLOCKED BY ALPHA-BTX

COUTURIER, S; BERTRAND, D; MATTER, JM; HERNANDEZ, MC; BERTRAND, S; MILLAR, N; VALERA, S; ... BALLIVET, M; + view all (1990) A NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT (ALPHA-7) IS DEVELOPMENTALLY REGULATED AND FORMS A HOMOOLIGOMERIC CHANNEL BLOCKED BY ALPHA-BTX. NEURON , 5 (6) 847 - 856.

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Abstract

cDNA and genomic clones encoding alpha-7, a novel neuronal nicotinic acetylcholine receptor (nAChR) alpha subunit, were isolated and sequenced. The mature alpha-7 protein (479 residues) has moderate homology with all other alpha and non-alpha nAChR subunits and probably assumes the same transmembrane topology. Alpha-7 transcripts transiently accumulate in the developing optic tectum between E5 and E16. They are present in both the deep and the superficial layers of E12 tectum. In Xenopus oo-cytes, the alpha-7 protein assembles into a homo-oligomeric channel responding to acetylcholine and nicotine. The alpha-7 channel desensitizes very rapidly, rectifies strongly above -20 mV, and is blocked by alpha-bungarotoxin. A bacterial fusion protein encompassing residues 124-239 of alpha-7 binds labeled alpha-bungarotoxin. We conclude that alpha-bungarotoxin binding proteins in the vertebrate nervous system can function as nAChRs.

Type: Article
Title: A NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT (ALPHA-7) IS DEVELOPMENTALLY REGULATED AND FORMS A HOMOOLIGOMERIC CHANNEL BLOCKED BY ALPHA-BTX
Keywords: CENTRAL NERVOUS-SYSTEM, BUNGAROTOXIN-BINDING-SITES, IMMUNOHISTOCHEMICAL LOCALIZATION, FUNCTIONAL EXPRESSION, MONOCLONAL-ANTIBODIES, XENOPUS OOCYTES, HIGH-AFFINITY, GENE FAMILY, MUSCLE, BRAIN
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/64467
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