Orie, NN and Vallance, P and Jones, DP and Moore, KP (2005) S-nitroso-albumin carries a thiol-labile pool of nitric oxide, which causes venodilation in the rat. AM J PHYSIOL-HEART C , 289 (2) H916 - H923. 10.1152/ajpheart.01014.2004.
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It is now established that S-nitroso-albumin (SNO-albumin) circulates at low nanomolar concentrations under physiological conditions, but concentrations may increase to micromolar levels during disease states (e.g., cirrhosis or endotoxemia). This study tested the hypothesis that high concentrations of SNO-albumin observed in some diseases modulate vascular function and that it acts as a stable reservoir of nitric oxide (NO), releasing this molecule when the concentrations of low-molecular-weight thiols are increased. SNO-albumin was infused into rats to increase the plasma concentration from <50 nmol/ l to similar to 4 mu mol/l. This caused a 29 +/- 6% drop in blood pressure, 20 +/- 4% decrease in aortic blood flow, and a 25 +/- 14% reduction of renal blood flow within 10 min. These observations were in striking contrast to those of an infused arterial vasodilator (hydralazine), which increased aortic blood flow, and suggested that SNO-albumin acts primarily as a venodilator in vivo. This was confirmed by the observations that glyceryl trinitrate (a venodilator) led to similar hemodynamic changes and that the hemodynamic effects of SNO-albumin are reversed by infusion of colloid. Infusion of N-acetylcysteine into animals with artificially elevated plasma SNO-albumin concentrations led to the rapid decomposition of SNO-albumin in vivo and reproduced the hemodynamic effects of SNO-albumin infusion. These data demonstrate that SNO-albumin acts primarily as a venodilator in vivo and represents a stable reservoir of NO that can release NO when the concentrations of low-molecular-weight thiols are elevated.
|Title:||S-nitroso-albumin carries a thiol-labile pool of nitric oxide, which causes venodilation in the rat|
|Keywords:||S-nitrosothiols, cardiac physiology, N-acetylcysteine, HUMAN PLASMA, GLYCERYL TRINITRATE, GAS-CHROMATOGRAPHY, MASS-SPECTROMETRY, N-ACETYLCYSTEINE, SERUM-ALBUMIN, GLUTATHIONE, NITROGLYCERIN, BLOOD, BIOACTIVATION|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of) > Metabolism and Experimental Therapeutics|
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