Wood, P and Caldwell, DE and Evans, E and Jones, M and Korber, DR and Wolfhaardt, GM and Wilson, M and Gilbert, P (1998) Surface-catalysed disinfection of thick Pseudomonas aeruginosa biofilms. J APPL MICROBIOL , 84 (6) 1092 - 1098.
Full text not available from this repository.
Transition metal catalysts were incorporated into polymers which formed the surface for bacterial attachment and biofilm formation in a constant depth film fermenter (100 mu m thickness), flow chamber (about 30 mu m thickness) and in batch culture (< 30 mu m thickness). The catalysts drive the breakdown of persulphates to reactive oxygen species. When Pseudomonas aeruginosa biofilms were exposed to dilute solutions of potassium monopersulphate (20 mu g ml(-1)-1 mg ml(-1)), significant enhancement of killing was notable for catalyst-containing surfaces over that of controls. The degree of enhancement was greatest for thin films, but was nevertheless significant for the 100 mu m thick biofilms. Fluorescence probes and viability staining, in conjunction with laser confocal microscopy, showed that reactive species were generated at the biofilm-substratum interface and killed the biofilm from the inside. Reaction-diffusion limitation now concentrates the active species within the biofilm rather than protecting it, and a diffusion pump is established whereby further treatment agent is drawn to the substratum enabling relatively thick biofilms to be disinfected.
|Title:||Surface-catalysed disinfection of thick Pseudomonas aeruginosa biofilms|
|Keywords:||INDWELLING DEVICES, BACTERIA|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases|
Archive Staff Only: edit this record