Albert, JT and Winter, H and Schaechinger, TJ and Weber, T and Wang, X and He, DZZ and Hendrich, O and Geisler, HS and Zimmermann, U and Oelmann, K and Knipper, M and Gopfert, MC and Oliver, D (2007) Voltage-sensitive prestin orthologue expressed in zebrafish hair cells. J PHYSIOL-LONDON , 580 (2) 451 - 461. 10.1113/jphysiol.2007.127993.
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Prestin, a member of the solute carrier (SLC) family SLC26A, is the molecular motor that drives the somatic electromotility of mammalian outer hair cells (OHCs). Its closest reported homologue, zebrafish prestin (zprestin), shares similar to 70% strong amino acid sequence similarity with mammalian prestin, predicting an almost identical protein structure. Immunohistochemical analysis now shows that zprestin is expressed in hair cells of the zebrafish ear. Similar to mammalian prestin, heterologously expressed zprestin is found to generate voltage-dependent charge movements, giving rise to a non-linear capacitance (NLC) of the cell membrane. Compared with mammalian prestin, charge movements mediated by zprestin display a weaker voltage dependence and slower kinetics; they occur at more positive membrane voltages, and are not associated with electromotile responses. Given this functional dissociation of NLC and electromotility and the structural similarity with mammalian prestin, we anticipate that zprestin provides a valuable tool for tracing the molecular and evolutionary bases of prestin motor function.
|Title:||Voltage-sensitive prestin orthologue expressed in zebrafish hair cells|
|Keywords:||MOTOR PROTEIN, COCHLEAR AMPLIFIER, MECHANOSENSORY NEURONS, MOTILITY, AMPLIFICATION, MEMBRANE, GENE, ELECTROMOTILITY, GENERATION, EMBRYOS|
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