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Ethanol stimulates apolipoprotein B mRNA editing in the absence of de novo RNA or protein synthesis

Giangreco, A; Sowden, MP; Mikityansky, I; Smith, HC; (2001) Ethanol stimulates apolipoprotein B mRNA editing in the absence of de novo RNA or protein synthesis. BIOCHEM BIOPH RES CO , 289 (5) 1162 - 1167. 10.1006/bbrc.2001.6082.

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Abstract

Apolipoprotein B (apoB) mRNA editing involves a site-specific modification of cytidine to form uridine. The reaction is catalyzed in the nucleus by a multiprotein editosome. Rat hepatic editing is regulated during development, metabolically and in response to ethanol. Ethanol stimulated editing in hepatocytes within minutes of exposure. In the present study, we show that ethanol stimulated apoB mRNA synthesis and apoB mRNA editing. Significantly, the proportion of edited apoB mRNA also increased following ethanol treatment of transcription or translation arrested cells. These data suggested that ethanol could regulate editing activity using pre-existing editosomal proteins. In addition, the presence of a suppressor of apoB mRNA editing activity was suggested by the finding that inhibition of mRNA or protein synthesis alone was sufficient to increase the proportion of edited RNA. It is proposed that the level of editing activity observed in hepatocytes may be the end result of positive and negative regulatory proteins. (C) 2001 Elsevier Science.

Type: Article
Title: Ethanol stimulates apolipoprotein B mRNA editing in the absence of de novo RNA or protein synthesis
DOI: 10.1006/bbrc.2001.6082
Keywords: MESSENGER-RNA, CATALYTIC SUBUNIT, MOORING SEQUENCE, HEPATIC EXPRESSION, MOLECULAR-CLONING, ENZYME APOBEC-1, RAT HEPATOCYTES, COMPLEX, MODULATION, INHIBITION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
URI: http://discovery.ucl.ac.uk/id/eprint/61800
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