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Production of Human α<inf>1</inf>-Antitrypsin in The Milk of Transgenic Sheep and Mice: Targeting Expression of cDNA Sequences to The Mammary Gland

McClenaghan, M; Archibald, AL; Harris, S; Simons, JP; Whitelaw, CBA; Wilmut, I; Clark, AJ; (1991) Production of Human α<inf>1</inf>-Antitrypsin in The Milk of Transgenic Sheep and Mice: Targeting Expression of cDNA Sequences to The Mammary Gland. Animal Biotechnology , 2 (2) pp. 161-176. 10.1080/10495399109525756.

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Abstract

Transgenic sheep have been produced carrying a gene construct comprising cDNA sequences encoding human alpha-l-antitrypsin (α1 AT) inserted into the first exon of the ovine β-lactoglobulin gene (BLG). Human α1 AT was detected in the milk of two out of three transgenic sheep and had a similar electrophoretic mobility to that of human plasma-derived α1 AT; however the levels of expression were low (18 μg/ml) and considered insufficient for commercial exploitation. This construct behaved in a similar manner in transgenic mice. A second construct, analogous to BLG-α1 AT, but containing the BLG cDNA was evaluated in transgenic mice. Low levels of expression were obtained, suggesting that such insertion constructs are not compatible with high level expression whatever the origin of the cDNA sequences. © 1991, Taylor & Francis Group, LLC. All rights reserved.

Type: Article
Title: Production of Human α<inf>1</inf>-Antitrypsin in The Milk of Transgenic Sheep and Mice: Targeting Expression of cDNA Sequences to The Mammary Gland
DOI: 10.1080/10495399109525756
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: http://discovery.ucl.ac.uk/id/eprint/61254
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