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CHARACTERIZATION OF THE ARG7 GENE OF CHLAMYDOMONAS-REINHARDTII AND ITS APPLICATION TO NUCLEAR TRANSFORMATION

PURTON, S; ROCHAIX, JD; (1995) CHARACTERIZATION OF THE ARG7 GENE OF CHLAMYDOMONAS-REINHARDTII AND ITS APPLICATION TO NUCLEAR TRANSFORMATION. EUR J PHYCOL , 30 (2) 141 - 148.

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Abstract

The Chlamydomonas reinhardtii gene ARG7 encoding the enzyme argininosuccinate lyase is a popular marker for the nuclear transformation of this alga. We present the complete nucleotide sequence of ARG7. The gene spans 7 kilobases and is split by 12 introns containing highly repetitive microsatellite elements. Molecular analysis of eight different arg7 mutants indicates that none of the mutations is the result of gross rearrangements at the ARG7 locus. We describe our analysis of factors influencing transformation efficiency and the construction of a cosmid library of the Chlamydomonas genome in a customised vector carrying the ARG7 marker. This library should greatly facilitate the cloning of Chlamydomonas genes by complementation.

Type: Article
Title: CHARACTERIZATION OF THE ARG7 GENE OF CHLAMYDOMONAS-REINHARDTII AND ITS APPLICATION TO NUCLEAR TRANSFORMATION
Keywords: ARG7, CHLAMYDOMONAS, COSMID LIBRARY, NUCLEAR TRANSFORMATION, GREEN-ALGA CHLAMYDOMONAS, INSERTIONAL MUTAGENESIS, NITRATE REDUCTASE, FLAGELLAR GENES, DNA, EXPRESSION, CRYSTALLINS, SEQUENCES
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/57634
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