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Retroviral infection of the FGF2 gene into MCF-7 cells induces branching morphogenesis, retards cell growth and suppresses tumorigenicity in nude mice

Liu, D; Buluwela, L; Ali, S; Thomson, S; Gomm, JJ; Coombes, RC; (2001) Retroviral infection of the FGF2 gene into MCF-7 cells induces branching morphogenesis, retards cell growth and suppresses tumorigenicity in nude mice. European Journal of Cancer , 37 (2) 268 - 280.

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Abstract

FGF2 (basic fibroblast growth factor) is a multifunctional growth factor and exhibits diverse function in different cell types. In breast, loss of FGF2 expression is associated with malignant progression. In order to understand the role of FGF2 in maintenance of normal breast structures and control of cell growth, we restored FGF2 expression in the breast cancer cell line MCF-7. The FGF2 retrovirally infected MCF-7 cells (MCF-7.F2.5) not only expressed FGF2 in cytoplasm and nuclei, but also released FGF2 into culture medium both on plastic and in Matrigel conditions. The MCF-7.F2.5 cells formed branches in Matrigel and this effect was abolished by the addition of a neutralising anti-FGF2 antibody or function blocking antibodies to alpha2, alpha3 and beta1 integrins. Furthermore, MCF-7.F2.5 cells lost their ability for anchorage-independent growth in soft agar. When MCF-7 and MCF-7.F2.5 cells were injected into nude mice, there was a 1.6- to 3.2-fold reduction of tumour volume with MCF-7.F2.5 cells in comparison with the parental MCF-7 cells. MCF-7.F2.5 cells also demonstrated a reduction in oestrogen receptor-alpha (ERalpha) both in vitro and in vivo. Our results suggest that introduction of the FGF2 gene into MCF-7 cells altered the malignant tumour cells towards a more benign phenotype in vitro and in vivo

Type:Article
Title:Retroviral infection of the FGF2 gene into MCF-7 cells induces branching morphogenesis, retards cell growth and suppresses tumorigenicity in nude mice
Additional information:UI - 21094773 LA - eng RN - 0 (Genetic Vectors) RN - 103107-01-3 (Fibroblast Growth Factor 2) PT - Journal Article DA - 20010222 IS - 0959-8049 SB - IM CY - England
Keywords:ABILITY, ANCHORAGE INDEPENDENT GROWTH, Animal, antibodies, Antibody, BENIGN, Blotting, Northern, branching, Breast, BREAST CANCER, Breast Neoplasms, BREAST-CANCER, cancer, Carcinogenicity Tests, cell, Cell Division, cell growth, Cell Line, Cell Size, Cell Type, CELL-LINE, CELLS, comparison, Condition, control, culture, Culture Media, Cytoplasm, Enzyme-Linked Immunosorbent Assay, expression, Female, Fibroblast, Fibroblast Growth Factor, Fibroblast Growth Factor 2, FIBROBLAST-GROWTH-FACTOR, Flow Cytometry, function, GENE, genetic, Genetic Vectors, genetics, growth, GROWTH FACTOR, GROWTH-FACTOR, IM, in vitro, in vivo, In-vitro, in-vivo, INDUCE, Infection, Integrin, Integrins, LA, LINE, loss, maintenance, Malignant, media, metabolism, mice, Mice, Nude, Morphogenesis, oestrogen, ORDER, Phenotype, plastic, prevention & control, PROGRESSION, REDUCTION, Result, Retroviridae, Role, soft, Structure, Support, Non-U.S.Gov't, SUPPRESSES, Tumour, tumour cell, VECTOR, VECTORS, VITRO, vivo, volume
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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