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SEPARATION AND CONCENTRATION OF BACTERIA WITH IMMOBILIZED ANTIBODY FRAGMENTS

MOLLOY, P; BRYDON, L; PORTER, AJ; HARRIS, WJ; (1995) SEPARATION AND CONCENTRATION OF BACTERIA WITH IMMOBILIZED ANTIBODY FRAGMENTS. J APPL BACTERIOL , 78 (4) 359 - 365.

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Abstract

New methods to quantitatively remove bacteria from food and water samples are required to meet modern safety standards. The recent development of techniques to make Fab/Fv/scFv fragments in bacteria has provided the opportunity to exploit antibodies as specialized chemicals for affinity removal technologies. Single-chain fragments against Pseudomonas aeruginosa have been expressed in Escherichia roll, purified via a fused poly-histidine tail and immobilized upon polystyrene beads. The resulting immunoaffinity columns have been shown to effectively remove greater than 90% of an applied 10 million bacteria after a single passage through the column. Column material in the absence of single-chain retained less than 10% of the bacteria. Pseudomonas were also removed from milk, mixed bacterial cultures and when present at low cell densities.

Type: Article
Title: SEPARATION AND CONCENTRATION OF BACTERIA WITH IMMOBILIZED ANTIBODY FRAGMENTS
Keywords: ESCHERICHIA-COLI, FV-FRAGMENTS, SINGLE, WATER
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Epidemiology and Health Care
UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Epidemiology and Health Care > Epidemiology and Public Health
URI: http://discovery.ucl.ac.uk/id/eprint/54879
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