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Rat chromaffin cells lack P2X receptors while those of the guinea-pig express a P2X receptor with novel pharmacology

Liu, M; Dunn, PM; King, BF; Burnstock, G; (1999) Rat chromaffin cells lack P2X receptors while those of the guinea-pig express a P2X receptor with novel pharmacology. BRIT J PHARMACOL , 128 (1) 61 - 68.

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Abstract

1 Whole-cell patch-clamp recording was used to determine the functional expression and pharmacological properties of P2X receptors in chromaffin cells dissociated from adrenal medullae of rats and guinea-pigs.2 In rat chromaffin cells maintained in culture for 1-7 days, ATP and UTP failed to evoke any detectable response.3 Guinea-pig chromaffin cells responded to ATP (100 mu M) with a. rapidly activating inward current. The amplitude of the response to ATF increased over the period cells were maintained in culture and so did the number of cells giving a detectable response, with 69%, of cells responding after greater than or equal to 4 days of culture.4 The response to ATP desensitized slowly, and had a reversal potential of 2.5 mV. The EC50 for ATP was 43 mu M. The potency order for ATP analogues was 2-MeSATP> ATP>ADP. Adenosine, UTP and alpha,beta-meATP were inactive.5 Suramin (100 mu M) and Cibacron blue (50 mu M) inhibited the ATP (100 mu M)-activated current by 51 and 47%, respectively. PPADS antagonized the response to ATP (100 mu M) With an IC50 of 3.2 mu M6 The ATP concentration-response curve shifted to the left at pH 6.8 (EC50, 19 mu M) and right at pH 8.0 (EC50, 96 mu M), without changing the maximal response. Zn2+ inhibited the response to ATP (100 mu M) with an IC50 of 48 mu M.7 This study indicates that expression of ATP-gated cation channels in chromaffin cells is species dependent. The P2X receptors in guinea-pig chromaffin cells show many characteristics of the P2X(2) receptor subtype.

Type: Article
Title: Rat chromaffin cells lack P2X receptors while those of the guinea-pig express a P2X receptor with novel pharmacology
Keywords: chromaffin, ATP, P2X, suramin, PPADS, cibacron blue, pH, Zn2+, GATED ION CHANNELS, ROOT GANGLION NEURONS, ATP-ACTIVATED CURRENT, EXTRACELLULAR PH, CATECHOLAMINE SECRETION, PHEOCHROMOCYTOMA CELLS, MYENTERIC NEURONS, CELIAC NEURONS, RELEASE, CALCIUM
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/53785
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