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Rapid monitoring of virus-like particles using an optical biosensor: A feasibility study

Tsoka, S; Gill, A; Brookman, JL; Hoare, M; (1998) Rapid monitoring of virus-like particles using an optical biosensor: A feasibility study. J BIOTECHNOL , 63 (2) 147 - 153.

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Abstract

Virus-like particles (VLPs) are multimeric proteins expressed by Saccharomyces cerevisiae. The particles are approximate to 80 nm in diameter and they are used as a framework for a range of biological products; for example as carriers of viral antigens. Rapid monitoring of purified VLPs was investigated using an optical biosensor. The aim was to develop an assay which may be employed for real-time bioprocess monitoring of VLPs. Problems of mass transfer of analyte were overcome through selection of a planar biosensor surface, in preference to the traditional polymer-coated surface. To prolong the surface activity for interaction analysis, a sandwich assay was developed which involved the use of a secondary capture species. It was shown that VLP concentration in pure solution could be determined within 10 min. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

Type: Article
Title: Rapid monitoring of virus-like particles using an optical biosensor: A feasibility study
Keywords: virus-like particle, biosensor, bioprocessing, rapid monitoring, YEAST, TY1, EXPRESSION, PROTEINS, SURFACE, TY-1
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/53629
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