UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks

Baharoglu, Z; Bradley, AS; Le Masson, M; Tsaneva, I; Michel, B; (2008) ruvA Mutants that resolve Holliday junctions but do not reverse replication forks. PLoS Genetics , 4 (3) , Article e1000012 . 10.1371/journal.pgen.1000012. Green and gold open access

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
485Kb

Abstract

RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which occurs at inactivated replication forks by the annealing of blocked leading and lagging strand ends. RuvAB was recently proposed to bind replication forks and directly catalyze their conversion into HJs. We report here the isolation and characterization of two separation-of-function ruvA mutants that resolve HJs, based on their capacity to promote conjugational recombination and recombinational repair of UV and mitomycin C lesions, but have lost the capacity to reverse forks. In vivo and in vitro evidence indicate that the ruvA mutations affect DNA binding and the stimulation of RuvB helicase activity. This work shows that RuvA's actions at forks and at HJs can be genetically separated, and that RuvA mutants compromised for fork reversal remain fully capable of homologous recombination.

Type:Article
Title:ruvA Mutants that resolve Holliday junctions but do not reverse replication forks
Open access status:An open access publication. A version is also available from UCL Discovery.
DOI:10.1371/journal.pgen.1000012
Publisher version:http://dx.doi.org/10.1371/journal.pgen.1000012
Language:English
Additional information:© 2008 Baharoglu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences

View download statistics for this item

Archive Staff Only: edit this record