Kukwikila, M and Howorka, S (2010) Electrically sensing protease activity with nanopores. J PHYS-CONDENS MAT , 22 (45) , Article 454103. 10.1088/0953-8984/22/45/454103.
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The enzymatic activity of a protease was electrically detected using nanopore recordings. A peptide substrate was tethered to microscale beads, and cleavage by the enzyme trypsin released a soluble fragment that was electrophoretically driven through the alpha-hemolysin protein pore, leading to detectable blockades in the ionic current. Owing to its simplicity, this approach to sense enzymatic activity may be applied to other proteases.
|Title:||Electrically sensing protease activity with nanopores|
|Keywords:||DNA ANALYSIS, ALPHA-HEMOLYSIN, PEPTIDES, PORE, IDENTIFICATION, COMPLEXES, MOLECULES, TRANSPORT, MEMBRANE, CHANNELS|
|UCL classification:||UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry|
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