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Electrically sensing protease activity with nanopores

Kukwikila, M; Howorka, S; (2010) Electrically sensing protease activity with nanopores. J PHYS-CONDENS MAT , 22 (45) , Article 454103. 10.1088/0953-8984/22/45/454103.

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Abstract

The enzymatic activity of a protease was electrically detected using nanopore recordings. A peptide substrate was tethered to microscale beads, and cleavage by the enzyme trypsin released a soluble fragment that was electrophoretically driven through the alpha-hemolysin protein pore, leading to detectable blockades in the ionic current. Owing to its simplicity, this approach to sense enzymatic activity may be applied to other proteases.

Type: Article
Title: Electrically sensing protease activity with nanopores
DOI: 10.1088/0953-8984/22/45/454103
Keywords: DNA ANALYSIS, ALPHA-HEMOLYSIN, PEPTIDES, PORE, IDENTIFICATION, COMPLEXES, MOLECULES, TRANSPORT, MEMBRANE, CHANNELS
UCL classification: UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Chemistry
URI: http://discovery.ucl.ac.uk/id/eprint/440920
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