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Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2

Kastning, K; Kukhtina, V; Kittler, JT; Chen, G; Pechstein, A; Enders, S; Lee, SH; ... Haucke, V; + view all (2007) Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. P NATL ACAD SCI USA , 104 (8) 2991 - 2996. 10.1073/pnas.0611170104.

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Abstract

alpha-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)type glutamate receptors undergo constitutive and ligand-induced internalization that requires dynamin and the clathrin adaptor complex AP-2. We report here that an atypical basic motif within the cytoplasmic tails of AMPA-type glutamate receptors directly associates with mu 2-adaptin by a mechanism similar to the recognition of the presynaptic vesicle protein synaptotagmin 1 by AP-2. A synaptotagmin 1-derived AP-2 binding peptide competes the interaction of the AMPA receptor subunit GluR2 with AP-2 mu and increases the number of surface active glutamate receptors in living neurons. Moreover, fusion of the GluR2-derived tail peptide with a synaptotagmin 1 truncation mutant restores clathrin/AP-2-dependent internalization of the chimeric reporter protein. These data suggest that common mechanisms regulate AP-2-dependent internalization of pre- and postsynaptic membrane proteins.

Type: Article
Title: Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2
DOI: 10.1073/pnas.0611170104
Keywords: endocytosis, postsynaptic, sorting signal, synaptic plasticity, SYNAPTIC-TRANSMISSION, MEDIATED ENDOCYTOSIS, TRAFFICKING, PLASTICITY, INTERNALIZATION, SYNAPTOTAGMIN, LTD, AP2, PHOSPHORYLATION, MECHANISMS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/437615
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