UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Release of reactive oxygen and nitrogen species from contracting skeletal muscle cells.

Pattwell, DM; McArdle, A; Morgan, JE; Patridge, TA; Jackson, MJ; (2004) Release of reactive oxygen and nitrogen species from contracting skeletal muscle cells. Free Radic Biol Med , 37 (7) pp. 1064-1072. 10.1016/j.freeradbiomed.2004.06.026.

Full text not available from this repository.

Abstract

A number of studies have indicated that exercise is associated with an increased oxidative stress in skeletal muscle tissue, but the nature of the increased oxidants and sites of their generation have not been clarified. The generation of extracellular reactive oxygen and nitrogen species has been studied in myotubes derived from an immortalized muscle cell line (H-2k(b) cells) that were stimulated to contract by electrical stimulation in culture. Cells were stimulated to contract with differing frequencies of electrical stimulation. Both induced release of superoxide anion and nitric oxide into the extracellular medium and caused an increase in extracellular hydroxyl radical activity. Increasing frequency of stimulation increased the nitric oxide generation and hydroxyl radical activity, but had no significant effect on the superoxide released. Additions of inhibitors of putative generating pathways indicated that contraction-induced NO release was primarily from neuronal NO synthase enzymes and that the superoxide released is likely to be generated by a plasma membrane-located, flavoprotein oxidoreductase system. The data also indicate that peroxynitrite is generated in the extracellular fluid of muscle during contractile activity.

Type: Article
Title: Release of reactive oxygen and nitrogen species from contracting skeletal muscle cells.
Location: United States
DOI: 10.1016/j.freeradbiomed.2004.06.026
Keywords: Animals, Catechols, Cell Line, Cytochromes c, Hydroxybenzoates, Mice, Muscle Cells, Muscle Contraction, Nitrates, Nitric Oxide Synthase, Nitrites, Oxidation-Reduction, Reactive Nitrogen Species, Reactive Oxygen Species, Salicylic Acid
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Developmental Neurosciences Prog
URI: http://discovery.ucl.ac.uk/id/eprint/43406
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item