UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Human muscle precursor cell regeneration in the mouse host is enhanced by growth factors.

Brimah, K; Ehrhardt, J; Mouly, V; Butler-Browne, GS; Partridge, TA; Morgan, JE; (2004) Human muscle precursor cell regeneration in the mouse host is enhanced by growth factors. Hum Gene Ther , 15 (11) pp. 1109-1124. 10.1089/hum.2004.15.1109.

Full text not available from this repository.

Abstract

The aim of this study was to optimize human muscle formation in vivo from implanted human muscle precursor cells. We transplanted donor muscle precursor cells (MPCs) prepared from postnatal or fetal human muscle into immunodeficient host mice and showed that irradiation of host muscle significantly enhanced muscle formation by donor cells. The amount of donor muscle formed in cryodamaged host muscle was increased by exposure of donor cells to growth factors before their implantation into injured host muscle. Insulin-like growth factor type I (IGF-I) significantly increased the amount of muscle formed by postnatal human muscle cells, but not by fetal human MPCs. However, treatment of fetal muscle cells with IGF-I, in combination with basic fibroblast growth factor and plasmin, significantly increased the amount of donor muscle formed. In vivo, human MPCs formed mosaic human-mouse muscle fibers, in which each human myonucleus was associated with a zone of human sarcolemmal protein spectrin.

Type: Article
Title: Human muscle precursor cell regeneration in the mouse host is enhanced by growth factors.
Location: United States
DOI: 10.1089/hum.2004.15.1109
Keywords: Animals, Cell Transplantation, Fibroblast Growth Factor 2, Growth Substances, Humans, Insulin-Like Growth Factor I, Lamin Type A, Mice, Muscle, Skeletal, Regeneration, Spectrin, Time Factors
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Developmental Neurosciences Prog
URI: http://discovery.ucl.ac.uk/id/eprint/43404
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item