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Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors

Braun, T; Backmann, N; Vogtli, M; Bietsch, A; Engel, A; Lang, HP; ... Hegner, M; + view all (2006) Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors. BIOPHYS J , 90 (8) 2970 - 2977. 10.1529/biophysj.105.072934.

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Abstract

Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteoliposomes using ink jet spotting; 2), successful detection of the prosthetic retinal removal (bleaching) from the bacteriorhodopsin protein by measuring the induced nanomechanical surface stress change; and 3), the quantitative response thereof, which depends linearly on the amount of removed retinal. Our results show this technique to be a potential tool to measure membrane protein-based receptor-ligand interactions and conformational changes.

Type:Article
Title:Conformational change of bacteriorhodopsin quantitatively monitored by microcantilever sensors
DOI:10.1529/biophysj.105.072934
Keywords:NANOMECHANICAL CANTILEVER ARRAY, ATOMIC-FORCE MICROSCOPY, INDUCED DEFLECTIONS, TARGET VALIDATION, ESCHERICHIA-COLI, PURPLE MEMBRANE, HYDROXYLAMINE, RESOLUTION, DNA, SPECTROSCOPY
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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