Lightbody, KL and Renshaw, PS and Collins, ML and Wright, RL and Hunt, DM and Gordon, SV and Hewinson, RG and Buxton, RS and Williamson, RA and Carr, MD (2004) Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility. FEMS MICROBIOL LETT , 238 (1) 255 - 262. 10.1016/j.femsle.2004.07.043.
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Abstract
We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
| Type: | Article |
|---|---|
| Title: | Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility |
| DOI: | 10.1016/j.femsle.2004.07.043 |
| Keywords: | CFP-10, ESAT-6, tuberculosis, protein-protein interaction, ESAT-6 family members, T-CELL ANTIGENS, COMPARATIVE GENOMICS, ESAT-6 FAMILY, ATTENUATION, VIRULENCE, BACILLUS, VACCINES, CFP-10, YEAST, BCG |
| UCL classification: | UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Infection and Immunity (Division of) |
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