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Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility

Lightbody, KL; Renshaw, PS; Collins, ML; Wright, RL; Hunt, DM; Gordon, SV; ... Carr, MD; + view all (2004) Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility. FEMS MICROBIOL LETT , 238 (1) 255 - 262. 10.1016/j.femsle.2004.07.043.

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Abstract

We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Type:Article
Title:Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility
DOI:10.1016/j.femsle.2004.07.043
Keywords:CFP-10, ESAT-6, tuberculosis, protein-protein interaction, ESAT-6 family members, T-CELL ANTIGENS, COMPARATIVE GENOMICS, ESAT-6 FAMILY, ATTENUATION, VIRULENCE, BACILLUS, VACCINES, CFP-10, YEAST, BCG
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Infection and Immunity (Division of)

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