Sajnani, G; Aricescu, AR; Jones, EY; Gallagher, J; Alete, D; Stoker, A; (2005) PTP sigma promotes retinal neurite outgrowth non-cell-autonomously. Journal of Neurobiology , 65 (1) 59 - 71.
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The receptor-like protein tyrosine phosphatase (RPTP) PTP sigma controls the growth and targeting of retinal axons, both in culture and in ovo. Although the principal actions of PTP sigma have been thought to be cell-autonomous, the possibility that RPTPs related to PTP sigma also have non-cell-autonomous signaling functions during axon development has also been supported genetically. Here we report that a cell culture substrate made from purified PTP sigma ectodomains supports retinal neurite outgrowth in cell culture. We show that a receptor for PTP sigma must exist on retinal axons and that binding of PTP sigma to this receptor does not require the known, heparin binding properties of PTP sigma. The neurite-promoting potential of PTP sigma ecto- domains requires a basic amino acid domain, previously demonstrated in vitro as being necessary for ligand binding by PTP sigma. Furthermore, we demonstrate that heparin and oligosaccharide derivatives as short as 8mers, can specifically block neurite outgrowth on the PTP sigma substrate, by competing for binding to this same domain. This is the first direct evidence of a non-cell-autonomous, neurite-promoting function of PTP sigma and of a potential role for heparin-related oligosaccharides in modulating neurite promotion by an RPTP. (c) 2005 Wiley Periodicals, Inc
|Title:||PTP sigma promotes retinal neurite outgrowth non-cell-autonomously|
|Additional information:||WoS ID: 000231989000006 JOCT|
|Keywords:||2005, A, acid, AMINO, amino acid, Amino-acid, AND, Axons, BINDING, BOTH, c, cell, cell culture, CONTROL, CULTURE, development, DOMAIN, FOR, FUNCTION, GROWTH, Heparin, in vitro, IN-VITRO, IS, LIGAND, OF, OLIGOSACCHARIDES, Periodicals, PROTEIN, Protein-Tyrosine-Phosphatase, RECEPTOR, report, Substrate, SUPPORT, THE, Tyrosine|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Child Health > Department of Neurosciences and Mental Health > ICH - Neural Development Unit|
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