UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Absence of antibodies to glutamate receptor type 3 (GluR3) in Rasmussen encephalitis

Watson, R; Jiang, Y; Bermudez, I; Houlihan, L; Clover, L; McKnight, K; Cross, JH; ... Lang, B; + view all (2004) Absence of antibodies to glutamate receptor type 3 (GluR3) in Rasmussen encephalitis. Neurology , 63 (1) pp. 43-50.

Full text not available from this repository.

Abstract

Objective: To determine the prevalence of serum antibodies to the ionotropic glutamate receptor 3 (GluR3) in patients with Rasmussen encephalitis ( RE), a severe epileptic disorder, and to compare with serum from control subjects and patients with intractable epilepsy (IE). Methods: The authors looked for serum immunoglobulin (Ig) G antibodies to GluR3 in 30 patients with RE, including two patients who had plasma exchange and 12 who had been treated with IV Igs with varying results, and 49 patients with IE and 23 healthy individuals, using ELISA with GluR3B peptide, Western blot analysis of recombinant full- length GluR3, immunoprecipitation of [S-35]- and [I-125]- labeled GluR3 extracellular domains, immunohistochemistry on rat brain sections, and electrophysiology of GluR3 expressed in Xenopus oocytes. Results: Low levels of antibodies to the GluR3B peptide were detected using ELISA in only 4 of the 79 patients with epilepsy ( 2 with RE and 2 with IE); binding to GluR3B in other sera was shown to be nonspecific. One other patient with IE had antibodies to recombinant GluR3 on Western blot analysis. However, none of the sera tested precipitated either the [S-35]- or the [I-125]-labeled GluR3 domains; none bound to rat brain sections in a manner similar to rabbit antibodies to GluR3; and none of the nine sera tested affected the electrophysiologic function of GluR3. Conclusions: GluR3 antibodies were only infrequently found in Rasmussen encephalitis or intractable epilepsy

Type: Article
Title: Absence of antibodies to glutamate receptor type 3 (GluR3) in Rasmussen encephalitis
Additional information: WoS ID: 000222812700010 JournalJUL 13839VJNEUROLOGY
Keywords: 12, 13, 2, 3, 4, A, Analysis, AND, Antibodies, Antibody, BINDING, BRAIN, CONTROL, DISORDER, DOMAIN, Electrophysiology, ENCEPHALITIS, Epilepsy, FOR, FUNCTION, HAD, HEALTHY, ie, Immunohistochemistry, INDIVIDUALS, JOURNAL, LENGTH, LEVEL, Low, METHODS, Neurology, OF, Oocytes, Other, PATIENT, patients, PEPTIDE, PLASMA, plasma exchange, PREVALENCE, rabbit, RAT, RAT BRAIN, RAT-BRAIN, RECEPTOR, Result, serum, THE, Xenopus
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Developmental Neurosciences Prog
URI: http://discovery.ucl.ac.uk/id/eprint/36790
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item