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Pre-ulcerative villous contraction and microvascular occlusion induced by indomethacin in the rat jejunum: A detailed morphological study

Anthony, A; Dhillon, AP; Thrasivoulou, C; Pounder, RE; Wakefield, AJ; (1995) Pre-ulcerative villous contraction and microvascular occlusion induced by indomethacin in the rat jejunum: A detailed morphological study. ALIMENT PHARM THERAP , 9 (6) 605 - 613.

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Abstract

Background: In indomethacin-induced jejunal ulceration in the rat, villi undergo both early microvascular injury and shortening that may involve activation of villous smooth muscle.Aim: This study sought to substantiate light microscopic observations using three-dimensional imaging of early villous architectural changes in response to indomethacin.Methods: At both 2 and 6 h after oral indomethacin 15 mg/kg or vehicle to groups of rats, the vasculature of the small intestine was visualised by both carbon-ink perfusion/confocal microscopy and injection casting. The mucosa was also examined for lesions by dissection microscopy and scanning electron microscopy.Results: In indomethacin-dosed rats examined by scanning electron microscopy and histology, the mucosa at 2 h showed villous shortening and wrinkling of the surface epithelium without epithelial loss; at 6 h, the mucosa was flattened, often with epithelial loss to expose a 'contracted' villous core. Examination of the vasculature in carbon-injected tissues indicated significant reductions of both mucosal height and inter-capillary distance at both 2 and 6 h post-indomethacin, Scanning electron microscopy of injection casts at 2 and 6 h indicated similar changes. These changes were not seen in control tissues.Conclusion: Histology, confocal microscopy and scanning electron microscopy support the proposal that villous shortening with disruption of surface capillary architecture are early changes in the ulcerative enteropathy induced by nonsteroidal anti-inflammatory drugs.

Type:Article
Title:Pre-ulcerative villous contraction and microvascular occlusion induced by indomethacin in the rat jejunum: A detailed morphological study
Keywords:PLATELET-ACTIVATING-FACTOR, SMALL-INTESTINE, GASTRIC DAMAGE, ENDOTHELIN, INJURY, FIBROBLASTS, ACID
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Pathology
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)

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