Agu, O; Hamilton, G; Baker, DM; Dashwood, MR; (2002) Endothelin receptors in the aetiology and pathophysiology of varicose veins. European Journal of Vascular and Endovascular Surgery , 23 (2) 165 - 171.
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Introduction: varicose veins are tortuous and poorly contractile. Their aetiology remains unclear. Neovascularisation has been suggested as a possible explanation. Endothelins are mitogenic, promoting proliferation and migration of endothelial cells via endothelin-B receptors. We hypothesise that endothelial cells and endothelin receptor density and distribution may play a role in the development of varicosis. Methods: saphenous vein segments from nine patients with varicose veins were compared to six controls. Slide- mounted sections were incubated in radioactive labelled endothelin-1 and receptor subtype-selective ligands and binding sites assessed using auto radiography. Endothelin-1 and endothelial cells were identified by immunohistochemistry and CD31-positive staining cells counted. Results: radioactive labelled endothelin-1 and endothelin-B receptor binding was reduced in varicose compared to control veins (p=0.04). Endothelin-A receptor binding was diffuse, with no difference in density in both groups (p=0.58). Endothelin-B receptor binding was diffuse with superimposed clusters. Although the density of medial endothelin-B receptor binding was reduced in the varicose group, more clusters were identified in this group compared to controls (p=0.005). CD-31 staining identified these clusters as endothelial cells. Conclusion: the reduced endothelin-1 binding and endothelin-B receptor density may be partially responsible for the reduced vasocontractility in varicose veins. We speculate that the increase in endothelin-B receptor binding CD31-positive endothelial cells in varicose veins may potentially stimulate mitogenesis and migration, leading to new vessel formation
|Title:||Endothelin receptors in the aetiology and pathophysiology of varicose veins|
|Additional information:||Journal English Article W B SAUNDERS CO LTD FEB 526AV LONDON EUR J VASC ENDOVASC SURG 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND|
|Keywords:||aetiology, endothelin, Endothelin Receptor, Endothelin Receptors, pathophysiology, Receptor, receptors, Varicose Veins, vein, Veins, As, BINDING, Binding Sites, BINDING-SITE, BINDING-SITES, cell, CELLS, CLUSTER, CLUSTERS, CO, Collagen, control, DENSITIES, DENSITY, development, difference, disease, distribution, Endothelial, endothelial cell, endothelial cells, ENDOTHELIAL-CELLS, endothelin 1, endothelin-1, Endothelin-B Receptor, Endothelins, English, ET(A), formation, groups, HUMAN BLOOD-VESSELS, Immunohistochemistry, INCREASE, LIGAND, ligands, LONG SAPHENOUS-VEIN, May, Methods, migration, NEOVASCULARIZATION, Patient, patients, play, proliferation, radiography, RD, Reflux, Result, Saphenous Vein, SECTIONS, SEGMENT, SITE, SITES, Staining, SURG, Varicose, VARICOSE-VEINS, VASCULAR TONE|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)|
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